Coding

Part:BBa_M31784:Design

Designed by: Emilienne Repak   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-28)


Gene VIII w/o Gene III Promoter + w/ mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

I had to remove the Gene III Promoter. Then I had to try to add the mCherry onto the extracellular side of protein VIII, which I believe is the N-terminus, which I think is coded on the 5' side of the gene, so I added mCherry before Gene VIII.


Source

M13K07 and the Standard Registry of Parts, thanks to work done in Roger Tsien's laboratory at UCSD.

References